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2 edition of glycerol operon of streptomyces coelicolor found in the catalog.

glycerol operon of streptomyces coelicolor

F. J. Davies

glycerol operon of streptomyces coelicolor

transcription initiation, termination and mRNA processing

by F. J. Davies

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  • 31 Currently reading

Published by UMIST in Manchester .
Written in English


Edition Notes

StatementF.J. Davies ; supervised by C.P. Smith.
ContributionsSmith, C. P., Biochemistry.
ID Numbers
Open LibraryOL21239631M

  The pathway for glycerol catabolism in Streptomyces coelicolor is determined by the gylABX operon. The sequence of about base-pairs (bp) preceding the structural genes of the operon has been determined, and related to a detailed transcriptional analysis of this by: Cloning and transcription analysis of the entire glycerol utilization (gylABX) operon of Streptomyces coelicolor A3(2) and identification of a closely associated Cited by:

Genetic mapping of the chromosome of Streptomyces coelicolor A3(2) began with the analysis of haploid recombinants selected from matings. A physical map of the S. coelicolor chromosome has recently been constructed, using pulsed-field gel electrophoresis of AseI and DraI fragments. The genetic map was found to be reasonably congruent with the physical map over the well-marked by: 2. Bacterial small RNAs are involved in post-transcriptional regulation. Using deep sequencing S. coelicolor transcriptome was analysed at the end of exponential growth. 63 small RNAs were identified. Expression of 11 of them was confirmed by Northern blot. The sRNAs were shown to be only present in Streptomyces species.. sRNA scr (Streptomyces coelicolor sRNA ) is located in the Family: Streptomycetaceae.

Transcription start sites and processing sites of the Streptomyces coelicolor A3(2) rrnA operon have been investigated by a combination of in vivo and in vitro transcription analyses. The data from these approaches are consistent with the existence of four in vivo transcription sites, corresponding to the promoters P1-P4. The transcription start sites are located at −, −, − and Cited by: Streptomyces coelicolor is the prototype for the investigation of antibiotic-producing and differentiating and a facilitator protein of the major intrinsic protein family may internalize glycerol. Of the predicted gene the same operon (18, 39, 42). This has been demonstrated byCited by:


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Glycerol operon of streptomyces coelicolor by F. J. Davies Download PDF EPUB FB2

Glycerolphosphate dehydrogenase (gylB) mutations (which cause glycerol sensitivity), and presumed gylcerol kinase (gylA) and/or regulatory mutations eliminating both glycerolphosphate dehydrogenase and glcerol kinase activities, map close to the argA locus of Streptomyces coelicolor Cited by: The glycerol utilization operon of Streptomyces coelicolor: genetic mapping of gyl mutations and the analysis of cloned gylDNA.

Seno ET, Bruton CJ, Chater by: The pathway for glycerol catabolism in Streptomyces coelicolor is determined by the gylABX operon. The sequence of about base-pairs (bp) preceding the structural genes of the operon has been determined, and related to a detailed transcriptional analysis of this by: The pathway for glycerol catabolism In Streptomyces coelicolor is determined by the gylCABX operon, which is transcribed from two closely spaced glycerol‐inducible, glucose‐repressible promoters.

Glycerol operon of streptomyces coelicolor book (or catabolite) repression of gyl is known to be exerted by a general catabolite repression system In which the soluble glucose kinase plays a central by: Glycerol operon regulatory protein.

Gene. gylR. Organism. Streptomyces coelicolor (strain ATCC BAA / A3(2) / M) Status. Reviewed-Annotation score: Glycerol metabolism, Transcription, Transcription regulation Names & Taxonomy i.

Protein names i: Recommended name: Glycerol operon regulatory protein. In S. coelicolor, genes for glycerol utilization form an operon, gylCABX, encoding a putative glycerol transporter, a glycerol kinase, a glycerolphosphate dehydrogenase and a gene of unknown.

Genes for glycerol utilization in Streptomyces coelicolor form an operon, gylCABX (15, 16), containing a gene for a putative glycerol transporter, a glycerol kinase, a glycerolphosphate dehydrogenase, and a gene of unknown function. They are preceded by gylR (5), which encodes a glycerol-inducible re-pressor controlling both gylR and the Cited by:   Genes for glycerol utilization in Streptomyces coelicolor form an operon, gylCABX (15, 16), containing a gene for a putative glycerol transporter, a glycerol kinase, a glycerolphosphate dehydrogenase, and a gene of unknown by: Streptomyces coelicolor A3(2) is the model species for the study of actinomycete genetics and biology, and a large array of genetic tools, including its genome sequence, is available to understand and manipulate the organism (Kieser et al., ; Bentley et al., ).

Strep-Cited by: The glyeerol operon of S. X, is tran- scribed from tandem glycerol-inducible promoters. gylPl/P2 (Smith and Chater. The gylR gene, situ- ated immediately upstream from the gyl promoters (Smith and Chater. encodes the specific repressor for the operon Cited by: Detailed transcriptional analysis of the S.

coelicolor glycerol (gylABX) operon and the nt sequence of an upstream region containing the putative regulatory gene gyIR and the gyIR- gylABX intercistronic region have been published (Smith and Chater, b).Cited by:   Streptomyces coelicolor is the prototype for the investigation of antibiotic-producing and differentiating actinomycetes.

As soil bacteria, streptomycetes can metabolize a wide variety of carbon sources and are hence vested with various specific permeases. Their activity and regulation substantially determine the nutritional state of the cell and, therefore, influence morphogenesis and Cited by: The pathway for glycerol catabolism in Streptomyces coelicolor is determined by the gylCABX operon, which is transcribed from two closely spaced glycerol-inducible, glucose-repressible.

Summary. The entire glycerol utilization (gylABX) operon of Streptomyces coelicolor A3(2) was cloned and its transcriptional organization and regulation was analyzed by Northern blotting, S 1 nuclease mapping and transcriptional ription of the operon is glycerol-inducible and glucose-repressible; gylA (presumptively encoding glycerol kinase), gylB (encoding sn-glycerol-3 Cited by:   Mutation of the crr-ptsI gene locus revealed that Streptomyces coelicolor uses the phosphotransferase system (PTS) for N-acetylglucosamineptsI, and ptsH, which encode the three general PTS phosphotransferases, are induced by N-acetylglucosamine but not by other PTSthe S.

coelicolor PTS is biased for N-acetylglucosamine utilization, a novel feature Cited by: Streptomyces lividans gyl DNA (for glycerol utilisation) was cloned by complementation of a Streptomyces coelicolorgyl mutant.

Restriction mapping showed that the cloned DNA was highly homologous (perhaps 99%) to S. coelicolor gyl DNA. Using phage-mediated mutational cloning, an internal fragment of the S. coelicolor gyl operon was used to generate a gyl mutant of S.

lividans, Cited by: 9. The dnaK operon of Streptomyces coelicolor contains four genes (5'-dnaK-grpE-dnaJ-hspR). The fourth gene encodes a novel heat shock protein, HspR, which appears so far to be unique to the high-G+C actinomycete group of by: In Streptomyces coelicolor and Streptomycesantibioticus, the phosphorolytic activity of PNPase is modulated by nucleoside diphosphates (11) and both phos-phorolysis and polymerization are modulated by the alarmone, (p)ppGpp (13).

As is the case in E. coli, the PNPase gene, pnp, is a part of an operon that includes the rpsO gene, whichCited by:   As another function of NdgR, we report the involvement of NdgR in glycerol metabolism in S.

coelicolor. Initially, a glycerol utilization operon containing gylCABX was found to be up-regulated in an ndgR deletion mutant (BG11) grown in N-acetylglucosamine solid minimal media compared with wild-type strain (M).Cited by: 3.

The pathway for glycerol catabolism In Streptomyces coelicolor is determined by the gylCABX operon, which is transcribed from two closely spaced glycerol-inducible, glucose-repressible promoters.

Abstract. Glucose kinase of Streptomyces coelicolor A3(2) is essential for glucose utilisation and is required for carbon catabolite repression (CCR) exerted through glucose and other carbon sources. The protein belongs to the ROK-family, which comprises bacterial sugar kinases and regulators.

To better understand glucose kinase function, we have monitored the cellular activity and Cited by: The entire glycerol utilization (gylABX) operon of Streptomyces coelicolor A3(2) was cloned and its transcriptional organization and regulation was analyzed by Northern blotting, S1 nuclease mapping and transcriptional by: A region of the Streptomyces coelicolor A3(2) chromosome was identified and cloned by using as a probe the lipase gene from Streptomyces exfoliatus M The cloned region consisted of bp, and carried a complete lipase gene, lipA, as well as a gene encoding a transcriptional activator (lipR).

The S. coelicolor A3(2) lipA gene encodes a functional extracellular lipase 82% identical to the S Cited by: